Efficacy of Second Prostaglandin F2 Alpha Treatment during Ovsynch Protocol on Pregnancy Rate Inseminated with Sex-sorted Semen in Buffaloes

Background: Ovsynch is one of the most adopted estrus synchronization protocols used for ovulation and fixed-time artificial insemination however, new corpus luteum (CL) induced by first GnRH treatment in inappropriately regressed with a single prostaglandin F2α. Inadequate CL regression reduces fertility. Considering this rationale, present research was implemented effect second F2 alpha during protocol on pregnancy rate inseminated sex-sorted semen buffaloes. Methods: Post-partum true anestrus multiparous buffaloes (n=24) were categorized as group I (Ovsynch; n=12) II (Double PG Ovsynch; n=12). Buffaloes from treated standard (D=7; G-P-G), while additional injection administered day eighth G-P-P-G) Ovsynch). All 16-20 hrs after last semen. Result: The signs exhibited numerically higher than I. service double group. addition improves quality increases rate.